AMBER Postdoctoral Fellowship: Next generation vitrification workflows for cryo-ET - Enabling high-throughput cryo-imaging across the microbial tree of life

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EMBL is Europe's life sciences laboratory - an intergovernmental organisation with more than 110 independent research groups and service teams covering the spectrum of molecular biology. It operates across six sites in Heidelberg (headquarters), Barcelona, Cambridge, Grenoble, Hamburg and Rome. Our mission is to perform basic research in molecular biology; train scientists, students and visitors at all levels; offer vital services to scientists in the public and private sectors within the member states; develop new instruments and methods; and engage actively in technology transfer. This post-doctoral position is part of the EU cofund research project AMBER, Advanced Multiscale Biological imaging using European Research infrastructures, will address scientific and sectoral gaps in biological imaging ranging from molecular, through cellular, to tissue, organ and organism levels of organisation, and is coordinated by LINXS Institute of advanced Neutron and X-ray Science. AMBER is funded by the EU Marie Skłodowska-Curie (MSCA) COFUND scheme. Around 8 postdocs will be recruited in the Final call with each fellowship lasting 36 months. AMBER has six core partners: Lund University/MAX IV, Sweden, the European Spallation Source (ESS), Sweden, the European Molecular Biology Laboratory (EMBL), Institut Laue-Langevin (ILL), France, the International Institute of Molecular Mechanisms and Machines, (IMOL), Poland, and the Leicester Institute of Structural and Chemical Biology, United Kingdom. Your work may include clinical and biomedical projects. It may also include technique development work aimed at combining imaging techniques and data analysis to provide a more integrated picture of life processes in the context of health and disease. To be a postdoc fellow at the AMBER programme you will get unprecedented medical, biological, and methodological capabilities, with a profound potential impact for Europe's next generation of research and researchers. When you have completed the AMBER programme you will be extraordinarily well equipped to further your career in academia, at infrastructures, in the health and MedTech sectors, and beyond. For more information about the total announced post-doctoral positions within in the AMBER co-fund project please visit https://www.euraxess.se/jobs/437869 The interviews will start in October/November 2026. For more information about AMBER, application and evaluation process etc please visit: ambercofund.eu Your role Cryo-electron tomography (cryoET) enables the visualization of cellular ultrastructure in a near-native state and provides structural information in situ, making it a powerful discovery tool for microbial cell biology. However, its application to a large variety of species in a high-throughput manner is hindered by the fact that sample preparation, i.e. vitrification and thinning by focused ion beam (FIB) milling, must be adapted for each sample type. A second major bottleneck is the limited number of samples that can be processed per experiment. Recent methodological advancements, such as micropatterning of grids and serial-lift out cryo-FIB-SEM, have greatly increased the throughput for mammalian cells and tissues. Similar technological breakthroughs are absent for bacteria, archaea and microbial eukaryotes. This project will address these challenges by developing next-generation sample preparation workflows that 1) standardize vitrification across diverse microbial species and 2) enable multiplexed preparation of different samples on a single EM grid. To this end, we propose to integrate microfluidic encapsulation of microbes with jet vitrification and (correlative) cryoFIB/ET imaging. The resulting workflow would allow high-throughput processing of phylogenetically and physiologically diverse microbes in a multiplexed manner, enabling comparative studies of cellular architecture at scale. You have a maximum 8 years after a doctoral degree (PhD), as required by the project Grant Agreement signed with the European Commission, at least one original publication in a peer-reviewed journal, a background in the relevant methods, a complete application package submitted through the AMBER portal (including CV and detailed research plan), and finally, strict compliance with the MSCA mobility rule that the researcher must not have resided or carried out his/her main activity (work, studies, etc.) in the host organisation's country for more than twelve months in the three years immediately prior to the call deadline. Contract length: 3 years Instructions on how to apply For more information and documents/templates/europass link, please visit https://www.ambercofund.eu/for-applicants Incomplete applications will not be considered. Please make sure that you upload all the required documents specified below i-ix. If you are interested in applying for more than one position (maximum 3) you must be prepared to make a full application for each individual position. Applicati

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