SRA, Flow Sorting

Responsibilities

• Conduct cell sorting experiments to isolate specific cell/nuclei populations, with high purity and viability.
• Operate and troubleshoot flow cytometry and cell sorting equipment
• Support the design and optimization of tissue dissociation and cell/nuclei isolation methods to meet project goals
• Perform the preparation and dissociation of human tissues and cells
• Perform nucleic acid extraction and sequencing library preparation.
• Work collaboratively with other team members across various functional areas.
• Maintain compliance with the Human Tissue Act (HTA) and COSHH regulations

Requirements

• Essential
• BSc or MSc degree in a biologically relevant field
• 3+ years' experience of flow cytometry (optimizing staining conditions, Improving signal/noise, panel optimization, reagent evaluation).
• Working knowledge of the principles of cell sorting
• An understanding of the maintenance, set up and troubleshooting of flow cytometry cell sorting equipment
• Proficiency in flow cytometry data analysis software and automated analysis pipelines
• Ability to identify opportunities for process improvement and laboratory automation within flow cytometry workflows
• Use of digital laboratory information management systems (Benchling preferred)
• Strong communication and collaborative skills with the ability to work effectively in a fast-paced startup environment
• Desirable
• Experience working within a flow cytometry and cell sorting facility
• Experience in isolation and sorting of nuclei
• Experience with optimization of tissue dissociation methods
• Experience working with multi-colour antibody panels for flow cytometry
• Experience and understanding of spectral flow cytometry, including unmixing and autofluorescence extraction
• Experience working with automation and understanding of how similar systems can be applied to cell dissociation and flow cytometry sorting workflows
• Experience with next generation sequencing library preparation and other downstream molecular biology processing
• Experience working with digital productivity tools such as Notion, Trello or Jira
• Experience using Benchling to create schemas, templates and automated workflows
• Knowledge and experience of working under HTA regulations
• Previous experience in a biotech or pharmaceutical industry setting
• Values and Behaviors:
• Encourage respectful disagreement and cultivate open-minded, ego-free interactions to continuously push each other towards excellence
• Seek out diverse perspectives; practice active listening and genuine curiosity to ensure all contributions are valued, regardless of source
• Recognize the impact of your behavior, language and attitudes, and strive for balanced, meaningful exchanges that enhance mutual growth and understanding in all interactions
• Use a company-first mindset to guide decision-making; prioritize team over individual success
• Take calculated risks and challenge convention in the quest for exceptional outcomes
• About Quotient:

Benefits

Additional Information

Position Summary: Quotient is seeking a Flow Cytometry and Fluorescence Activated Cell Sorting Senior Research Associate to join our High Throughput Platform team. The primary focus of this role is the isolation and sorting of cells and nuclei from human tissues to support downstream somatic genomics workflows, including DNA extraction and sequencing library preparation to support detection of disease relevant somatic variants. The successful candidate will work closely with our Flow Cytometry and Automation platforms to refine our processes and scale our Flow Cytometry operations. In addition, this position will provide growth opportunities to learn and contribute to disease projects across our histology and/or molecular workflows. The ideal candidate will thrive in a fast-paced, cross-functional environment, supporting delivery across prioritized workstreams with agility, positivity, and a proactive mindset. They will demonstrate initiative in solving problems, raising concerns, and driving improvements, while remaining committed to continuous learning, open feedback, and working beyond their comfort zone.

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