Research Specialist B/C

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University Overview The University of Pennsylvania, the largest private employer in Philadelphia, is a world-renowned leader in education, research, and innovation. This historic, Ivy League school consistently ranks among the top 10 universities in the annual U.S. News & World Report survey. Penn has 12 highly-regarded schools that provide opportunities for undergraduate, graduate and continuing education, all influenced by Penn's distinctive interdisciplinary approach to scholarship and learning. As an employer Penn has been ranked nationally on many occasions with the most recent award from Forbes who named Penn one of America's Best Large Employers in 2023. Penn offers a unique working environment within the city of Philadelphia. The University is situated on a beautiful urban campus, with easy access to a range of educational, cultural, and recreational activities. With its historical significance and landmarks, lively cultural offerings, and wide variety of atmospheres, Philadelphia is the perfect place to call home for work and play. The University offers a competitive benefits package that includes excellent healthcare and tuition benefits for employees and their families, generous retirement benefits, a wide variety of professional development opportunities, supportive work and family benefits, a wealth of health and wellness programs and resources, and much more. Posted Job Title Research Specialist B/C Job Profile Title Research Specialist C Job Description Summary A full-time Research Laboratory Manager position at the University of Pennsylvania's New Bolton Center is available in the Stewart laboratory in the Department of Clinical Studies. The role of this position is to be the primary research specialist within a laboratory focused on translational osteoarthritis research. The candidate should be highly motivated with a high level of competence and prior experience in cellular and molecular techniques. Job Description Research duties will include independent collection and preparation of animal tissues from cadavers for tissue harvest; ability to perform tissue processing and knowledge on storage techniques for intended analyses; distinct knowledge and experience with cell harvest and tissue culture of joint tissues using defined protocols as well as the development and validation of novel protocols; independent knowledge and competence in molecular analyses and sequencing of tissues including PCR, RNA isolation; preparation of tissues for '-omics' analyses, immunoassay techniques such as ELISA and flow cytometry; and competence with tissue assays including staining and fluorescence for cellular characterization. The candidate will be expected to produce and present data to the PI and other individuals within the laboratory on a regular basis as a part of weekly laboratory meetings. The candidate will be responsible for managing all equipment and certifications within the laboratory, and responsibilities will include ordering supplies for research and generating invoices for collaborative activities. The individual should have a collaborative mindset and a willingness to actively participate in the laboratory research team. Job Responsibilities Molecular analyses of tissues, including immunoassay techniques, PCR, sequencing, flow cytometry, tissue staining (including fluorescence) Tissue culture, including protocol development and optimization in species of interest, and cell line preservation Tissue harvest/collection and preparation for ex vivo studies, histology, or other analyses (-omics, sequencing, etc) from cadaver samples Collation of acquired data, graphical presentation, and limited analysis Laboratory management and administrative tasks, including supervising individuals working in the laboratory environment, ordering supplies, generating invoices, etc. Other duties and responsibilities as assigned

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