Research Technician II

Responsibilities

• Perform and support experiments involving human primary cell culture, including T cell isolation, activation, expansion and functional characterization
• Execute molecular and cellular assays, including flow cytometry, molecular biology techniques (e.g. PCR), gene delivery, genome editing and gene engineering approaches, and functional immune assays
• Support the establishment, optimization and implementation of experimental workflows and protocols
• Maintain laboratory organization, including reagent preparation, inventory management, equipment coordination and ordering
• Contribute to assay development, troubleshooting and quality control activities
• Assist in training students and new laboratory members
• Ensure compliance with laboratory safety, biosafety and regulatory standards
• BSc in immunology, biology, biotechnology, molecular biology or a related life sciences discipline; MSc is an asset
• Relevant laboratory experience in cell culture, immunology, molecular biology or related areas
• Hands-on experience with mammalian cell culture; experience with human primary immune cells and/or cell therapy manufacturing is highly desirable
• Experience with flow cytometry and basic molecular biology techniques
• Strong organizational skills and attention to detail
• Excellent documentation and communication skills
• Ability to work both independently and as part of a collaborative team
• Interest in translational immunology, cell therapy and immune engineering
• Why join UHN?
• Competitive offer packages
• Government organization and a member of the Healthcare of Ontario Pension Plan (HOOPP https://hoopp.com/ )
• Close access to Transit and UHN shuttle service
• A flexible work environment
• Opportunities for development and promotions within a large organization
• Additional perks (multiple corporate discounts including: travel, restaurants, parking, phone plans, auto insurance discounts, on-site gyms, etc.)
• Current UHN employees must have successfully completed their probationary period, have a good employee record along with satisfactory attendance in accordance with UHN's attendance management program, to be eligible for consideration.
• All applications must be submitted before the posting close date.
• UHN uses email to communicate with selected candidates. Please ensure you check your email regularly.
• Please be advised that a Criminal Record Check may be required of the successful candidate. Should it be determined that any information provided by a candidate be misleading, inaccurate or incorrect, UHN reserves the right to discontinue with the consideration of their application.
• UHN is an equal opportunity employer committed to an inclusive recruitment process and workplace. Requests for

Benefits

Additional Information

Union : Non-Union Number of Vacancies: 1 Site : PMCRT Department : Ajmera Transplant Centre Research Institute (ATC-RI) Reports to : Principal Investigator Salary Range : $31.02 - $38.78/hr Hours : 37.5 per week Status: Permanent Full-Time Closing Date: June 26, 2026 Position Summary Position summary: The successful candidate will join a research program in programmable immune engineering at UHN, based at the ATC-RI and affiliated with the University of Toronto. The lab focuses on designing engineered human immune cells that maintain stable and regulated function in complex clinical environments, with a particular emphasis on transplantation alongside applications in cancer and infection. The research program integrates CRISPR-based genome engineering, RNA-based modulation, and advanced human tissue and organoid models to define generalizable design principles for immune function under clinical stress conditions such as inflammation, immunosuppression, and chronic antigen exposure. A central goal is to establish programmable immune resilience as a design parameter for clinically deployable cell therapies. Experimental approaches include genetic engineering of human T cells, development of non-viral and mRNA-based delivery strategies, and functional validation in physiologically relevant human model systems, closely aligned with translational and biomanufacturing workflows at UHN. Example application areas include regulatory T cell engineering for enhanced stability under inflammatory and pharmacologic stress. The Research Technician II will play a central role in establishing and maintaining the wet lab infrastructure and supporting experimental workflows focused on human immune cell engineering and cell therapy research. The successful candidate will contribute to a growing translational research environment integrating genome engineering, RNA-based approaches, and advanced human model systems.

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